Dry ice shipping instructions
Options for insulated shipping boxes:
Permacool Insulated Foam Shipper Boxed Kit 1.5″ Thick Wall – 11.5″ x 10″ x 9.25″ https://www.amazon.com/Permacool-Insulated-Shipper-Boxed-Thick/dp/B00OD7ROQE
Insulated Foam Shipper Boxed Kit 1.5″ Thick Wall -11.5″ x 10″ x 9.25″ https://www.ebay.com/itm/Insulated-Foam-Shipper-Boxed-Kit-1-5-Thick-Wall-11-5-x-10-x-9-25-Inner-Dim-/272375409913
There are two main options, for which the prices may vary:
- Overnight shipping with less dry ice.
- 3-day-shipping with more dry ice.
Make sure to avoid shipping near weekends & holidays.
Fresh > Flash freeze (dry ice) > Slow freeze (freezer)
Air exposure will also kill many microbes in the stool. Do not let the stool come into contact with the toilet or any other non-sterile surfaces. The best way to do it is to go directly into a plastic ziplock bag like the one in this picture.
Various squatting positions should be adequate
You can also lean on a wall for support and balance. Flatten the air out before zipping it shut. If you are shipping only 1 sample, either bring it right away to buy dry ice and put it in the dry ice (should be fine at room temp or a insulated lunch bag for 1/2 an hour), or refrigerate (not freeze) until the dry ice is purchased. If you’re shipping multiple samples they’ll probably have to be slow frozen in the regular fridge.
Some recipients may request for the sample to be mixed with saline (salt water – do not use iodized salt) and/or glycerol (anti-freeze that protects the microbes from freezing damage). In these cases you could add those to the ziplock bag, zip shut, then mash/mix with your hand from the outside of the bag.
For dry ice:
You can try various web searches for “buy dry ice [your city]”. Example results:
There are some local grocery stores that have it.
This directory could work too to find a close provider: http://www.dryicedirectory.com/world.htm
To create saline:
Add 1 teaspoon of non-iodized salt to 2 cups of filtered or distilled water. Or 1/4th teaspoon of salt for 1 cup of water.
Glycerol is a laxative and thus may be problematic for some people. If the recipient decides they want you to use glycerol it should make up 10-15% of the total volume of added liquid. About 1 tablespoon of glycerol for every 9 tablespoons of saline.
Glycerol and glycerin are the same thing. Many department/grocery stores should carry it. Read the label and make sure the only ingredient is glycerol. It is also available online: